Toluene XYLAZINE HYDROCHLORIDE, also known as XYLAZINE HYDROCHLORIDE, is a kind of sedative veterinary drugs. XYLAZINE HYDROCHLORIDE can produce mild inhibition, abate functional activities, thereby play to eliminate restlessness, quiet recovery of a class of drugs. In recent years, individual animal husbandry breeders drive because of economic interests, be good at adding this kind of medicine in the process of livestock and poultry raising in order to have the effect of sedation and hypnosis, increasing weight and urging fat, shortening time out of the market; XYLAZINE HYDROCHLORIDE is used to feed livestock and poultry. In addition, in the process of animal transportation, in order to reduce the death and weight loss of animals and prevent the deterioration of meat quality, XYLAZINE HYDROCHLORIDE are often used to reduce the loss.
XYLAZINE HYDROCHLORIDE determination method
The residual tranquilizer veterinary drug is Zolpidem, haloperidol, chlorazepine hydrochloride, promazine hydrochloride, nitrazepam, chlorpromazine salt, perphenazine hydrochloride, fluphenazine hydrochloride, clonazepam, XYLAZINE HYDROCHLORIDE, propionylpromazine, carbazoline, acepromazine, floperidol, azapperon;
(1) Ground, mixed and homogenized mutton, stored in -18 ~ -40℃ for later use;
(2) the spare samples were thawed to room temperature, and 4.5 ~ 5.5g of mutton samples were accurately weighed into a 50mL polypropylene centrifuge tube, and 15ml of 10:90v/v ammonium-acetonitrile and 30μLβ -glucosiduridase ≥100000units/mL were added. The samples were placed in the dark, and then stirred at 35 ~ 40℃ for 8 ~ 12h. The supernatant was removed after ultrasonic extraction at 30 ~ 40℃ for 10 ~ 15min, centrifuged at 3500-4500 r/min for 5 ~ 10min, and 15mL10:90, V/V ammonium-acetonitrile was added into the residue. Ultrasonic extraction at 30 ~ 40℃ for 10 ~ 15min, centrifuged at 3500-4500 r/min for 5 ~ 10min. The supernatant was merged twice, 4.0-8.0g NaCl was added, and fully mixed. After standing for 3-10min, the supernatant was removed, decompressed and concentrated at 30-40 ℃ until it was nearly dry. 1mL0.027mol L-190:10V/V formic acid aqueous solution was added and acetonitrile was rotated to dissolve all residue in the bottle. After passing 0.22 ~ 0.45 μm organic phase filtration membrane, for analysis and determination;
(3) Liquid chromatography-tandem mass spectrometer detection: chromatographic column: Acquity UPLC CSHTM C18; Mobile phase: A was acetonitrile, B was 0.027mol L-1 formic acid aqueous solution, 0 ~ 15min, A10% → 70%, 15 ~ 16min, A70% → 10%, flow rate was 0.3 ~ 0.5mL min-1. Injection volume: 5-20 μL; ESI+ ionization mode; Multistage response detection (MRM); Capillary voltage: +3.0 ~ 3.5kV; Ion source temperature: 100 ~ 150℃; Dissolvent temperature: 300 ~ 380℃; Cone-hole gas flow rate: 40 ~ 55L H-1; Flow rate of desolvent gas: 450 ~ 550L H-1;
(4) Prepare matric matching standard working fluid of different concentrations, and perform step (3) in turn, perform linear regression of concentration by peak area, and obtain linear regression equation and correlation coefficient of tranquilizers;
(5) The chromatographic peak areas of the samples in steps (1) to (3) were substituted into the linear regression equation of the standard working liquid of tranquilizer veterinary drug matrix matching, and the detection results were obtained.
